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Видео в категории “Наука и техника”
16" Macbook Pro First Impressions!
 
07:07
Hands on with the 16" Macbook Pro, the newest best Apple laptop! MKBHD Merch: http://shop.MKBHD.com Video Gear I use: http://kit.com/MKBHD/video-gear#recom... Tech I'm using right now: https://www.amazon.com/shop/MKBHD Intro Track: Whoa Whoa Whoa - Watsky Playlist of MKBHD Intro music: https://goo.gl/B3AWV5 ~ http://twitter.com/MKBHD http://instagram.com/MKBHD http://facebook.com/MKBHD
Просмотров: 2816297 Marques Brownlee
This researcher created an algorithm that removes the water from underwater images
 
03:56
Why do all the pictures you take underwater look blandly blue-green? The answer has to do with how light travels through water. Derya Akkaynak, an oceangoing engineer, has figured out a way to recover the colorful brilliance of the deep. Read the full story at SciAm: https://www.scientificamerican.com/article/sea-thru-brings-clarity-to-underwater-photos/ Video by Erik Olsen: http://erikolsen.com/
Просмотров: 2216517 Scientific American
How to Talk to Aliens
 
26:07
Are we alone in the universe? Even if we could contact aliens, what would we say? How would we say it? And, most importantly, should we even be trying to make contact at all? This episode takes me on a journey to compose and send my own personal message into outer space. Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
The Origins of Disgust
 
12:44
With the help of Cecile Sarabian, Dr. Andrew MacIntosh and some fake poop, Michael observes levels of disgust among Japanese Macaques.
Просмотров: 508252 Vsauce
The danger of AI is weirder than you think | Janelle Shane
 
10:30
Visit http://TED.com to get our entire library of TED Talks, transcripts, translations, personalized Talk recommendations and more. The danger of artificial intelligence isn't that it's going to rebel against us, but that it's going to do exactly what we ask it to do, says AI researcher Janelle Shane. Sharing the weird, sometimes alarming antics of AI algorithms as they try to solve human problems -- like creating new ice cream flavors or recognizing cars on the road -- Shane shows why AI doesn't yet measure up to real brains. The TED Talks channel features the best talks and performances from the TED Conference, where the world's leading thinkers and doers give the talk of their lives in 18 minutes (or less). Look for talks on Technology, Entertainment and Design -- plus science, business, global issues, the arts and more. You're welcome to link to or embed these videos, forward them to others and share these ideas with people you know. For more information on using TED for commercial purposes (e.g. employee learning, in a film or online course), submit a Media Request here: http://media-requests.TED.com Follow TED on Twitter: http://twitter.com/TEDTalks Like TED on Facebook: http://facebook.com/TED Subscribe to our channel: http://youtube.com/TED
Просмотров: 624283 TED
Behavior and Belief
 
28:27
How can a lie become true? In this episode, Dr. Aaron Blaisdell and I create a game show that is actually a giant “human Skinner Box” to observe the formation of superstitious beliefs. And Dr. Samuel Veissière helps me design and perform a placebo reverse exorcism, harnessing the power of belief in both science and religion to convince normal people that a spirit has possessed their bodies. Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
Mind Reading
 
22:22
In this episode I visit a researcher who is studying memory by using machine learning and neuroimaging to detect and predict people's brain states. I also travel to Japan to meet with a team working on ways to record the content of peoples’ dreams. Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
The Fastest Charging Smartphone In The World
 
07:30
The Oppo Reno Ace is the fastest charging smartphone I've ever tested. The Reno Ace uses Oppo's new SuperVOOC 2.0 technology to charge the phone at 65 watt peak. SuperVOOC 2.0 is capable of recharging the Reno Ace from 0-100 in 30 minutes. FOLLOW ME IN THESE PLACES FOR UPDATES Twitter - http://twitter.com/unboxtherapy Facebook - http://facebook.com/unboxtherapy Instagram - http://instagram.com/unboxtherapy
Просмотров: 3484443 Unbox Therapy
Should I Die?
 
31:05
If I could live forever, should I? How does being reminded of our own mortality affect us psychologically? In this episode I speak with mortician and death positivity activist Caitlin Doughty and visit a cryonics facility trying to extend human life indefinitely. Will I take them up on their offer, or will I choose to die? Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
The Stilwell Brain
 
26:32
There are 100 billion individual neurons in the human brain. Working together, they allow us to make sense of, and move through, the world around us. Scientists have built replicas of the human brain with computers, but no one has ever successfully made a brain out of humans. On this episode, I’ll travel back to my hometown of Stilwell, Kansas, and turn it into a working brain! Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
The Stanford Prison Experiment
 
34:28
Normal people can become monsters given the right situation. That’s the standard narrative of the Stanford Prison Experiment, one of the most famous psychological experiments of all time. But what if the cause of its participants’ cruel behavior wasn’t what we’ve always been told?
Просмотров: Vsauce
Moral Licensing
 
28:28
How are our moral decisions influenced by factors we’re not aware of? A phenomenon known as Moral Licensing claims that when we do something good, we often subconsciously allow ourselves to then do something bad. In this episode, I take a look at whether those who donate money to charity become more likely to let a kid take the blame for a crime they know they committed. Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: Vsauce
The Cognitive Tradeoff Hypothesis
 
24:22
Humans are the only Earthlings with complex language. But at what cost was that ability acquired? In this episode, I visit Tetsuro Matsuzawa to learn about his influential cognitive tradeoff hypothesis. Available with YouTube Premium - https://www.youtube.com/premium/originals. To see if Premium is available in your country, click here: https://goo.gl/A3HtfP
Просмотров: 13810152 Vsauce
How we can eliminate child sexual abuse material from the internet | Julie Cordua
 
13:51
Visit http://TED.com to get our entire library of TED Talks, transcripts, translations, personalized Talk recommendations and more. Social entrepreneur Julie Cordua works on a problem that isn't easy to talk about: the sexual abuse of children in images and videos on the internet. At Thorn, she's building technology to connect the dots between the tech industry, law enforcement and government -- so we can swiftly end the viral distribution of abuse material and rescue children faster. Learn more about how this scalable solution could help dismantle the communities normalizing child sexual abuse around the world today. (This ambitious plan is part of the Audacious Project, TED's initiative to inspire and fund global change.) The TED Talks channel features the best talks and performances from the TED Conference, where the world's leading thinkers and doers give the talk of their lives in 18 minutes (or less). Look for talks on Technology, Entertainment and Design -- plus science, business, global issues, the arts and more. You're welcome to link to or embed these videos, forward them to others and share these ideas with people you know. For more information on using TED for commercial purposes (e.g. employee learning, in a film or online course), submit a Media Request here: http://media-requests.TED.com Follow TED on Twitter: http://twitter.com/TEDTalks Like TED on Facebook: http://facebook.com/TED Subscribe to our channel: http://youtube.com/TED
Просмотров: 62965 TED
Genome Editing with CRISPR-Cas9
 
04:13
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. Feng Zhang, a leader in the development of this technology, is a faculty member at MIT, an investigator at the McGovern Institute for Brain Research, and a core member of the Broad Institute. Further information can be found on Prof. Zhang’s website at http://zlab.mit.edu . Images and footage courtesy of Sputnik Animation, the Broad Institute of MIT and Harvard, Justin Knight and pond5.
Просмотров: 2100378 McGovern Institute
Plantar Fasciitis Stretches
 
03:18
Judi Bar, Yoga Program Manager at Cleveland Clinic, shows you how to prevent or treat plantar fasciitis with a series of plantar fasciitis stretches. ➨ Visit Cleveland Clinic: http://bit.ly/XlxDfr ➨ Visit Health Hub from Cleveland Clinic: http://bit.ly/VBQ3nW ➨ Subscribe to our YouTube Channel: http://bit.ly/W0bJ0y ➨ Like Cleveland Clinic on Facebook: http://on.fb.me/WMFkul ➨ Follow Cleveland Clinic on Twitter: http://bit.ly/Uua1Gs ➨ Follow Cleveland Clinic on Google+: http://bit.ly/136vcTe ➨ Follow Cleveland Clinic on Instagram: http://bit.ly/12gMABx ➨ Connect with Cleveland Clinic on LinkedIn: http://linkd.in/120XfNs ➨ Follow Cleveland Clinic on Pinterest: http://bit.ly/11QqS3A
Просмотров: 308018 Cleveland Clinic
7 Things to Try to Reduce Your Acid Reflux
 
02:03
Acid blockers may relieve heartburn. But they can't stop the epidemic of reflux in this country. Try these steps to figure out what may be causing your reflux, and how you may be able to reverse it. Download the Functional Medicine Treatment Guide: ccf.org/FMGetstarted
Просмотров: 206085 Cleveland Clinic
What is Dialectical behavior therapy for adolescents (DBT)?
 
08:02
Dialectical behavior therapy for adolescents and young adults (DBT) is a clinical program within the Young Adult and Family Center that targets high risk, multi-problem adolescents. We specialize in identifying and treating depression and risky behavior in adolescents, including self-injury, suicidal ideation and suicide attempts, substance use, binging and purging, risky sexual behavior, physical fighting, and other forms of risk-taking. DBT has been adapted by Alec Miller and Jill Rathus from Marsha Linehan’s initial conceptualization of DBT, which was developed for adults diagnosed with borderline personality disorder. Our adaptation follows the work of Miller and Rathus, who originally modified DBT for use with teens. Our program targets five areas: Confusion about self Impulsivity Emotional instability Interpersonal problems Parent-teen problems The treatment has been shown to be effective in treating self-harming adolescents with depression who demonstrate some traits of borderline personality disorder that are beyond that expected of typical adolescent development. Depression in adolescence is characterized by depressed or irritable mood, changes in appetite and sleep, withdrawal from and loss of interest in usual activities and friends, feelings of hopelessness and worthlessness, agitation and/or fatigue, difficulty concentrating, difficulty making decisions, and suicidal ideation. Some traits of borderline personality disorder that may be of concern to teens and parents include: unstable sense of self, unstable interpersonal relationships, inappropriate or uncontrollable anger or other emotions, serious mood swings, recurrent self-harm or and/or suicide attempts, chronic feelings of emptiness, and impulsivity that puts the teen at risk. Teens and young adults referred to the DBT Program typically have many or all of the problems listed above, which can lead to difficulties fulfilling their obligations in school and at home. This perpetuates their negative ideas about themselves.
Просмотров: 348910 UC San Francisco (UCSF)
DreamStation cleaning | Philips | CPAP Therapy Devices
 
02:46
Cleaning your DreamStation CPap Therapy Device See our stories of how Philips innovations are improving peoples' lives around the world at: http://philips.com/innovationandyou Connect with Philips Online: - Visit Philips Sleep and Respiratory Care Website: www.philips.com/healthcare/solutions/sleep-and-respiratory-care - Find Philips Respiratory Wellness on Facebook: https://www.facebook.com/philipsrespiratorywellness - Find Philips Better Sleep on Facebook: https://www.facebook.com/PhilipsSleepandRespiratoryCare - Follow Philips Sleep and Respiratory on Twitter: https://twitter.com/PhilipsResp - Find Philips Sleep and Respiratory on LinkedIn: https://www.linkedin.com/company/philipssleepandrespiratorycare/ - Find Philips Respiratory Showcase page on LinkedIn: https://www.linkedin.com/company/philips-respiratory - Learn Philips news: http://philips.to/1r8yRG5
Просмотров: 57685 Philips Healthcare
Ion Torrent™ next-gen sequencing technology
 
03:07
See how this groundbreaking technology works. http://www.lifetechnologies.com/iontorrent Leveraging consumer technology for scientific breakthroughs Ion Torrent™ technology takes an entirely new approach to sequencing, making it faster, simpler, and more affordable than ever before. Unlike other sequencing technologies, Ion Torrent™ systems sequence DNA using a semiconductor chip, similar to the chip found in your digital camera. While the chip in your camera has a sensing layer covered with millions of pixels that translate light into digital information, an Ion chip has millions of wells covering those pixels. Whenever a nucleotide is incorporated into a single strand of DNA, a hydrogen ion is released. This is how the Ion Torrent system sequences DNA, by reading this chemical change directly in the well on the chip. In essence, each well works as the world's smallest pH meter.
Просмотров: 210936 Thermo Fisher Scientific
DreamWear Gel Pillows Introduction | Philips
 
02:56
Connect with Philips Online: - Visit Philips Sleep and Respiratory Care Website: www.philips.com/healthcare/solutions/sleep-and-respiratory-care - Find Philips Respiratory Wellness on Facebook: https://www.facebook.com/philipsrespiratorywellness - Find Philips Better Sleep on Facebook: https://www.facebook.com/PhilipsSleepandRespiratoryCare - Follow Philips Sleep and Respiratory on Twitter: https://twitter.com/PhilipsResp - Find Philips Sleep and Respiratory on LinkedIn: https://www.linkedin.com/company/philipssleepandrespiratorycare/ - Find Philips Respiratory Showcase page on LinkedIn: https://www.linkedin.com/company/philips-respiratory - Learn Philips news: http://philips.to/1r8yRG5
Просмотров: 64292 Philips Healthcare
How TaqMan Works -- Ask TaqMan® Ep. 13 by Life Technologies
 
04:00
Submit your real-time PCR questions at http://www.lifetechnologies.com/asktaqman Just how does TaqMan work? Sr. Field Applications Specialist Doug Rains explores the specific mechanism by which TaqMan® achieves its unparalleled specificity and sensitivity. Around the world, researchers rely on TaqMan® for gene expression, SNP gentoyping , protein expression, pathogen detection and quantification, and more. For many years, TaqMan has been the gold-standard chemistry for real-time PCR. It's famed for its unparalleled specificity, sensitivity, and ease of use. So it's not surprising that users want to know what Shrikant at ICL College in India asked recently: namely, "How does TaqMan work?" I'm glad you asked. Just like any PCR, TaqMan-based reactions require a double-stranded template, as well as two fairly standard, target-specific primers. But unlike those used in regular PCR, TaqMan Assays require a third, sequence-specific oligo called a probe. TaqMan probes are quite different from the primers in two ways. First, they can't be extended by our friendly enzyme, Taq Polymerase, since they lack a free hydroxyl group. What's more, TaqMan probes are covalently joined to two other molecules. On the 5'-end, there's a fluorescent molecule known as the reporter -- called that because it reports signal to us as we generate more and more product. On the 3'-end is a molecule known as the quencher, which quenches the fluorescent signal from the reporter under certain circumstances. Let's see what those circumstances are. Here we're looking at an intact probe, with the reporter in green, the quencher in red. Normally, when we zap the probe with light, we expect the reporter to get excited and fluoresce. But because the quencher is in close proximity to the reporter, instead what happens is, the energy gets transferred from reporter to quencher. The transfer of energy is known as FRET, or Fluorescent Resonance Energy Transfer. The important thing to note here is that, as long as the probe remains intact, there is no permanent increase in fluorescent signal from the reporter. However, if the reporter and quencher are permanently separated during the reaction, and then light strikes the reaction, the Reporter does in fact fluoresce, producing signal the instrument can detect. The basic idea, then, is this: each time we create a new PCR amplicon, we want to permanently split the reporter and quencher. By doing so, florescence will always increase proportionally with product, allowing us to effectively monitor what's happening to our reactions throughout the run. Here it is in action. We begin our reactions (CLICK) by denaturing our template at a high temperature. As we lower the temperature, our probe and primers bind. Taq now comes in, finds the primers, and begins the extension phase of PCR by creating new complementary strands of DNA. But wait a second: there's a probe sitting in the way. It's a showdown in the making! What will the polymerase do? Stop in its tracks? Turn back in fear? Nay, friends, not Taq Polymerase. You see, our enzyme has what's referred to as "exonuclease activity." Meaning? It pretty much eats DNA for lunch. So when Taq reaches the probe, it simply chews it to bits on its way to creating new amplicon. As a result, the reporter and quencher are physically separated, creating a permanent increase in fluorescence that, not coincidentally, perfectly accords with our doubling of product. And, of course, that our real-time instrument can monitor and record this increase in fluorescence after each cycle, generating an amplification plot that's more than a little useful for interpreting our data.
Просмотров: 128454 Thermo Fisher Scientific
Cleveland Clinic Empathy - Cleveland Clinic Annual Report 2012
 
04:24
Cleveland Clinic tries to imagine feeling what the patient is feeling.
Просмотров: 70252 Cleveland Clinic
Immune Cell Migration in the Zebrafish Inner Ear
 
01:10
Immune cells within the perilymphatic space of the inner ear of several zebrafish embryos 80 hpf showing: MIP view of two immune cells (orange), one of which has ingested dextran particles (blue), before and after AO plus deconvolution for 438 time points at 13 sec intervals; volume rendered view in another embryo, showing a migrating immune cell and a dividing endothelial cell; and tracking of the position and velocity of an immune cell in a third embryo (c.f., Fig. 6E,F, figs. S13-15). Credit: T. Liu et al./Science 2018 Read more: https://www.hhmi.org/news/new-microscope-captures-detailed-3-d-movies-of-cells-deep-within-living-systems
Просмотров: 729706 HHMI Howard Hughes Medical Institute
More Than Medicine | Edward McKay, Jr. (Surgical Technician)
 
04:21
Ed McKay is an inspiration, to his friends, his colleagues and anyone who ever wanted to dream big. He started his career at Johns Hopkins 15 years ago in Environmental Services and worked his way up to surgical technician. He's worked side by side with some of the world's greatest surgeons and earned the prestigious Baker King Award. His story demonstrates the partnership between a great institution and the people who make it extraordinary.
Просмотров: 64796 Johns Hopkins Medicine
Motorola Razr hands on: The revived RAZR is a fashion-forward foldable
 
09:04
Motorola has resurrected its beloved Razr phone, a staple of mid-2000s cool, as a foldable device. The new Razr distinguishes itself from the Galaxy fold and Huawei's Mate X by folding vertically instead of horizontally. Unfolded, it looks like a regular smartphone, albeit one with a chunky "chin", but folded in half it's practically pint-sized, with a small front display to see your notifications, control media, or take a selfie. There's still a lot of questions when it comes to durability and foldables, but the new Razr feels extremely well constructed, and folds in half with no visible gap. It does have a few downsides, including a mid-range processor, single camera, and questionable battery capacity, but this is the first foldable we could see ourselves using as a daily device. Read our full story on Engadget: https://www.engadget.com/2019/11/13/motorola-razr-foldable-hands-on-preview-2019/ Get More Engadget: • Like us on Facebook: http://www.facebook.com/engadget • Follow us on Twitter: http://www.twitter.com/engadget • Follow us on Instagram: http://www.instagram.com/engadget • Read more: http://www.engadget.com
Просмотров: 5771615 Engadget
Osteosarcoma | Jaliyah's Story
 
03:15
At age 9, Jaliyah was diagnosed with an osteosarcoma. After swelling from an ankle sprain did not subside, a visit to Jaliyah’s primary care physician revealed that Jaliyah’s ankle pain was caused by a tumor. When local specialists recommended amputation, Jaliyah’s mother Joyce searched for second opinion to save her daughter’s leg. Joyce found Dr. Carol Morris, chief of Orthopaedic Oncology at The Johns Hopkins Hospital, who offered an alternative to amputation. Dr. Morris and her team performed a complex limb-sparing surgery allowing Jaliyah to keep her leg. Learn more about Jaliyah’s story and the care team that gave her the chance to continue dancing. To learn more about our orthopaedic services, please visit hopkinsmedicine.org/ortho. To schedule an appointment, call 443-997-2663 (BONE).
Просмотров: 374262 Johns Hopkins Medicine
Spinal Pathways 2 - Dorsal Column - Medial Lemniscus Pathway
 
04:36
http://www.handwrittentutorials.com - This is the second video in a series on the major pathways in the spinal cord. This video looks at the dorsal column - medial lemniscus pathway from the periphery to the cortex. For more entirely FREE tutorials and accompanying PDFs visit http://www.handwrittentutorials.com
Просмотров: 541742 Handwritten Tutorials
Pseudotumor Cerebri | Ashley's Story
 
04:42
After visiting 30+ physicians, Ashley was diagnosed with the rare condition known as pseudotumor cerebri. She and her family turned to the experts at Johns Hopkins who worked as a team to implant a stent, a new approach to treating this condition that is typically treated with a shunt. Find out more about our experts at www.hopkinsmedicine.org/neuro For appointments, Maryland residents should call 410-550-1470, and out-of-state residents should contact 1-855-884-6754.
Просмотров: 101031 Johns Hopkins Medicine
Understanding multiple sclerosis and what it does to the body
 
03:12
Multiple sclerosis (MS) is a disease of the central nervous system estimated to affect 2.3 million people worldwide. It is a chronic disease in which the immune system abnormally attacks the insulation and support around the nerve cells (myelin sheath) in the brain, spinal cord and optic nerves, causing inflammation and consequent damage. MS is a leading cause of non-traumatic disability in young people, usually striking between 20 and 40 years of age. There is no cure for MS, but research continues to better understand and treat the disease. To learn more about Roche and MS, visit http://www.roche.com/ms. Subscribe to our YouTube channel now: https://www.youtube.com/user/roche?sub_confirmation=1 Get in touch with us: https://www.roche.com/ https://www.facebook.com/RocheCareers https://www.linkedin.com/company/roche https://twitter.com/roche Roche has been committed to improving lives since the company was founded in 1896 in Basel, Switzerland. Today, Roche creates innovative medicines and diagnostic tests that help millions of patients globally. Roche is a leader in research-focused healthcare with combined strengths in pharmaceuticals and diagnostics. Roche is the world’s largest biotech company, with truly differentiated medicines in oncology, immunology, infectious diseases, ophthalmology and neuroscience. For more information and insights visit: https://www.roche.com/
Просмотров: 129304 Roche
Spinal Pathways 3 - Spinothalamic Tract
 
03:29
http://www.handwrittentutorials.com - This is the third video in a series on the major pathways in the spinal cord. This video looks at the Spinothalamic Tract from the periphery to the cortex. For more entirely FREE tutorials and accompanying PDFs visit http://www.handwrittentutorials.com
Просмотров: 546405 Handwritten Tutorials
Cancer: from a healthy cell to a cancer cell
 
04:40
In 5 minutes find out how and why a normal cell becomes a cancer cell: risk factors and treatment.
Просмотров: 721642 Sanofi
Ph.Diddy is on the scene - Animation of life in the biotech lab
 
04:50
See all Ph.Diddy videos here: - "Ph.Diva and the Mystery Band" - http://youtu.be/ryhSGUzzz8U - "Ph.Diddy at the Conference" - http://youtu.be/ajdbuj8P784 - "Behind the scened at Halloween with Ph.Diddy": http://youtu.be/r_NdyX70MF8 PCR Arnold Young (aka the Ph.Diddy) is a biotech Ph.D candidate. Arnold was an A+ student, and is now ready to take over the world of science! He soon discovers how life in the lab is filled with drive and devotion, frustrations and fulfillment, hard work, late hours, repeated experiments, peer review and a strive for respect and recognition. How will the ups and downs of life in the lab shape our Ph.Diddy on his journey to have his first scientific paper published? Acknowledgements: This video is inspired by all the dedicated everyday-heroes-of-science. Invitrogen recognizes your passion and admires your perseverance! BY POPULAR DEMAND - the song is now available on iTunes! http://itunes.apple.com/us/album/the-ph.diddy-is-on-the-scene/id478987896?i=478987900&ign-mpt=uo%3D4 For more information on PCR, please visit www.lifetechnologies.com/elevatepcr -------------------- Lyrics: I was the black belt of my class, marked out as an achiever. First day on the job, enter the gene-weaver. My future colleagues look up from the PCR machine, checking me out man. The Ph.Diddy's on the scene. So I claim my pipette and pimp it up to halt the theivin'. Get the thermocycler going, my samples cool chilling. Yeah, I shoot tips like I've been here all my life. Waiting for instrument time with my free pizza slice. I run my first Western blot. Man, this lab scene is easy. But wait up, hold a minute. I'm feeling kinda queasy. Is that Trizol I can smell? Oh, my head's in a spin! 've gone and dropped my freakin' sample in the biohaz bin. He's a super fly grad. Life plans to shape the future, that's right! Sees his name in lights and a centre spread in Nature. All right! I'm always splitting cells, always working late. I've cleared the biohazard bin so much I herniate. Got samples botched, samples lost, samples dropped on the floor. Messy benches, empty buffers, lab politics galore. My PI's buggin'. Man he sounds like my father. Western blot once again, but development shows nada. I'm doin' my time in the cell culture hood - at the weekend bro. It's the only time I could! Then suddenly my data is starting to make sense. Even Western blot bands start to make an appearance. So I repeat once again all the steps I need to follow. But every culture well... turned yellow. He's a super fly grad. Life plans to shape the future, that's right! Sees his name in lights and a centre spread in Nature. All right! I'm talkin' 'bout the ooh, ooh... Serum and media get swiped with no apology. And all my cells are showing abnormal morphology. So now my PI's freakin' "Find something you can prove!" But contamination aggravation knocks me off my groove. So I stay late once again as the lab detainee. Yeah, its 9 pm but the thermocycler's free I don't understand! What's happened now? That's it. Yeah, time to give up. I'm throwing in the towel. Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah! Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah! My transfection worked - confirmed with Pol II chIP - mutagenesis determined the TF targeted. I'm not going down! I can still do BioChem! My genes will express, I'm gonna beat it out of them. And yeah, I must have results that no one can undress. And quickly move my paper from in prep to in press. I'm finally finished writing the discussion, whilst my PI once again, "tweaks" my introduction. So, here we go, yo. Here's the scenario: Peer review's due to hit. When exactly? I don't know. My paper will be published. My research is legit. OK it's not quite Nature but, I think I nailed it! He's a super fly grad. Life plans to shape the future, that's right! Sees his name in lights and a centre spread in Nature. All right! He's a super fly grad. Life plans to shape the future, that's right! Sees his name in lights and a centre spread in Nature. All right! Ooh, ooh, supergrad. I'm talkin' 'bout the ooh, ooh... Super bad lab grad, yeah!
Просмотров: 330317 Thermo Fisher Scientific
Bloodhound LSR smashes 550mph target speed
 
02:45
Bloodhound LSR, today, reached a new top speed of 562mph / 904kmh as she ripped across the Kalahari Desert racing track as part of her high speed test programme. --------- We’d love to hear from you! Please let us know what you think or any questions you’d like answering in the comments section below. Don't forget to subscribe to our channel to never miss any of our exclusive videos about Bloodhound LSR: http://bit.ly/31C1RyT http://www.bloodhoundlsr.com/ https://www.instagram.com/Bloodhound_LSR/ https://www.facebook.com/BLOODHOUNDSSC/ https://twitter.com/Bloodhound_LSR https://www.linkedin.com/company/35648633/admin/
Просмотров: 195043 Bloodhound LSR
Worried About Carpal Tunnel? Try 3 Simple Stretches
 
00:51
Repetitive wrist stress from computer use, factory work or hobbies can cause numbness and tingling in your hands and wrists. Watch this demonstration of three simple stretches and recommendations that can bring relief from Andrew Bang, DC.
Просмотров: 484918 Cleveland Clinic
Endometriosis | Q&A with Dr. Patzkowsky
 
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Dr. Kristin Patzkowsky, a minimally invasive surgeon from the department of Gynecology and Obstetrics answers your common questions asked about endometriosis. Learn more at: http://www.hopkinsmedicine.org/gynecology_obstetrics/ Endometriosis is a benign disorder characterized by the presence of endometrial tissue (the tissue that lines the uterus) outside the uterine cavity where it becomes attached to reproductive or abdominal organs. The patches of endometrial tissue swell with blood during menstruation as if they were still in the uterus. Endometriosis is a common disorder, most prevalent between the ages of 25 and 40. Symptoms vary and are not strictly correlated with the severity of the disease; they may worsen with time, but tend to diminish during pregnancy and cease with menopause. Many women have no symptoms at all. Treatment depends on the severity of symptoms, the age of the woman, and whether she wishes to have children. Questions Answered: 1. What is endometriosis? 0:03 2. What are the symptoms of endometriosis? 0:14 3. Can endometriosis affect fertility? 1:15 4. What are the treatment options for endometriosis? 2:01 5. Can endometriosis be a recurring issue? 2:51 6. What is the difference between minimally invasive surgery and open surgery for endometriosis? 3:10 7. What type of physician should I see for surgical treatment of endometriosis? 4:23
Просмотров: 74979 Johns Hopkins Medicine
Fecal Microbial Transplantation: A Treatment for Clostridium Difficile
 
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When antibiotics kill off too many "good" bacteria in the digestive tract during treatment of C. Diff, Fecal Microbial Transplants can help replenish bacterial balance. For more information: http://www.hopkinsmedicine.org/gastroenterology_hepatology/clinical_services/advanced_endoscopy/fecal_transplantation.html
Просмотров: 108035 Johns Hopkins Medicine
Principles of PCR
 
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What are the key steps of PCR?
Просмотров: 55757 Thermo Fisher Scientific
What is an Exosome? (Episode 1)
 
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Episode 1 in a 6-part mini documentary series, see the full series here: http://www.lifetechnologies.com/exosomesdocumentary We asked 10 prominent scientists to share their thoughts on science and in particular the field of exosomes research. The video-series tell the story and history of this exciting new area of research, and its impact on other research fields such as cancer and immunology. The video-series also discusses the potential future therapeutic and diagnostic applications that may come from exosome research. Contributors: - Xandra Breakefield, Ph.D........... Professor, Massachusetts General Hospital - Jan Lötvall, MD., Ph.D .............. Professor, University of Gothenburg, President of ISEV - Suresh Mohla, Ph.D.................. Chief (TBMB) and Division Associate Director, NIH - Esther Nolte-'t Hoen, Ph.D......... Senior Scientist, Utrecht University - Michiel Pegtel, Ph.D................. Assistant Professor, VUmc, Amsterdam - Graça Raposo-Benedetti, Ph.D.. Director of Research, CNRS, Institut Curie - Phillip A. Sharp, Ph.D............... Nobel Laureate, Professor, MIT - Johan Skog, Ph.D.................... CSO, Exosomes Diagnostics - Dima Ter-Ovanesyan.................Ph.D Student, Harvard University - Clotilde Thery, Ph.D................. Director of Research, INSERM, Institut Curie, Secretary General of ISEV Acknowledgements: Members of the research groups of Michiel Pegtel (Cancer Center Amsterdam, VUmc), Graça Raposo-Benedetti (Structure and Membrane Compartments, CNRS, Institut Curie) and Clotilde Théry (Immunity and Cancer, INSERM, Institut Curie) Special thanks to the ISEV society for their strong contribution in leading the field of exosome research forward, and to the participants at the ISEV RNA Workshop in New York City October 1st-2nd 2012 Kudos to all scientists that have the courage to follow their curiosity! If you want to learn about the exosomes-specific products available from Life Technologies: http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/exosomes.html?cid=exoep1
Просмотров: 95602 Thermo Fisher Scientific
Mitochondrial Disease Patient Story - Cleveland Clinic Children's Hospital
 
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Mitochondrial disease affects 1 in 5,000 children. Learn more about the positive experience a Wooster, Ohio family has had in the treatment of mitochondrial disease for their two children at Cleveland Clinic Children's Hospital.
Просмотров: 100053 Cleveland Clinic
Using Standard Curve to Estimate DNA Quantity - Forensic Focus #4
 
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Submit your questions at http://www.thermofisher.com/forensicfocus Everyone wants to know how much DNA is in their extract, but then they ask: how can I tell if my estimate is accurate? The standard curve holds the answers. A standard curve is a tool that allows us to estimate the DNA concentration of unknown samples by comparing them to standards with known DNA concentrations. In this example, the standards consist of a 10-fold dilution series ranging from 50 ng/ul down to 5 pg/ul. During each PCR cycle, the amount of fluorescent signal for each standard in the dilution seies is measured. When the fluorescent signal crosses the detection threshold the cycle number is recorded as a Ct value, or threshold cycle value. The Ct value is what ultimately is used to create the standard curve. The Ct values are inversely proportional to the concentration of DNA in the standards. The high-concentration, 50 ng/ul standard will cross the detection threshold first, generating a “low” Ct. The low-concentration, 5 pg/ul standard will take many more cycles to cross the same threshold - and therefore the Ct will be higher. The Ct values for each dilution of the standard curve are plotted on a graph, and the software generates a regression line that fits the data. Because the standards are 10-fold dilutions, we expect the change in Ct from one standard to the next to be uniform. An uneven distribution of Ct values might indicate that the dilution series was not accurately pipetted. Let’s take a look at the standard curve for a specific DNA target, the small autosomal target. The X axis is the log of the known standard concentrations. The Y axis is the Ct value of each standard. Now, Do you see the quality metrics at the bottom of the screen? Let’s review Slope, Y intercept, and R2.? The slope measures the efficiency of the PCR reaction. In a perfect world, a slope of -3.3 indicates that the PCR reaction is 100% efficient; the target DNA is doubled each cycle. Two copies become four; four become eight; and so on. The Y intercept is the expected Ct value for a 1ng/ul sample. The R2 value measures how well the regression line fits the data points. A line that fits the data points perfectly has an R2 of 1. If your data points are scattered, the R2 value for the line will be lower. The Ct values of your standards affect the slope, the Y intercept, and the R2 value. It is very important to prepare the standard dilution series carefully to ensure consistent and accurate results! Running the standards in duplicate can help ensure you have a high quality standard curve. Once your standard curve passes the metrics test, it can be used to evaluate an unknown sample! The Ct value of the unknown sample is measured, and compared to the standard curve to estimate the DNA concentration of the unknown sample. Couldn’t be simpler! That’s it for today. If you have other questions, just click on the link below. And don’t forget- when in doubt, refer Back to the Bases!
Просмотров: 48072 Thermo Fisher Scientific
Johns Hopkins Surgeon: The Long Way Here - Kofi Boahene's Story
 
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Becoming a doctor at Johns Hopkins demands sacrifice. Some of our physicians have surprising histories. Kofi Boahene is one of three doctors with intriguing backgrounds featured in the Fall issue of Hopkins Medicine magazine. Read the full story http://www.hopkinsmedicinemagazine.org
Просмотров: 97188 Johns Hopkins Medicine
Meniscus Injuries | Q&A with Dr. Andrew Cosgarea
 
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Dr. Andrew Cosgarea, chief of the division of sports medicine at Johns Hopkins Orthopaedics, discusses meniscus injuries. He explains how meniscus injuries happen, the signs and symptoms and how they are treated, and how to prevent a meniscus injury. Learn more at: www.hopkinsmedicine.org/ortho Questions Answered: (0:01) What is a meniscus? (0:21) How does the meniscus get injured/torn? (0:53) What are the symptoms of a meniscal tear? (1:29) Does meniscus surgery cause, treat or prevent arthritis? (2:00) Once diagnosed, how are meniscal tears treated? (2:44) Does every meniscal tear require surgery? (3:14) What is the recovery after meniscal surgery? (4:03) What is articular cartilage, and what is so unique about it? (4:37) When can I return to sports after an injured meniscus? (5:30) Can the meniscus get torn a second time? (5:55) What are the different surgery options available for an injured meniscus?
Просмотров: 72971 Johns Hopkins Medicine
What Causes Cancer? Cancer Mutations and Random DNA Copying Errors
 
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Johns Hopkins Kimmel Cancer Center scientists report data from a new study providing evidence that random DNA copying “mistakes” account for nearly two-thirds of the mutations that cause cancer. Their research is grounded on a novel mathematical model based on DNA sequencing and epidemiologic data from around the world. More information on the research: http://www.cristiantomasetti.com/tvtheory/
Просмотров: 77645 Johns Hopkins Medicine
Your Night in the Sleep Laboratory
 
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A sleep study, also called a polysomnogram is an overnight study of your sleep, which takes place in a specialized laboratory. It is conducted by a trained technologist and is designed to detect problems with your sleep. The goal of the study is to have a natural night of sleep that is as uncomplicated and comfortable as possible. This video demonstrates the process and explains the reason for this procedure. Learn more: http://www.hopkinsmedicine.org/health/healthy-sleep/
Просмотров: 113158 Johns Hopkins Medicine
Johns Hopkins Medicine | Aplastic Anemia
 
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Aplastic anemia occurs when your bone marrow doesn’t make enough red and white blood cells, and platelets. The body's immune system is confused and begin to attack these critical performing cells. Learn more about aplastic anemia at: http://www.hopkinsmedicine.org/healthlibrary/conditions/adult/hematology_and_blood_disorders/aplastic_anemia_85,p00075/
Просмотров: 220557 Johns Hopkins Medicine
Pharmacokinetics 1 - Introduction
 
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http://www.handwrittentutorials.com - This tutorial is the first in the Pharmacokinetics series. It introduces the the four elements (ADME) of pharmacokinetics. This tutorial also discusses in detail the pharmacokinetic properties of drugs, such as Maximum Concentration, Tmax, and the drug Half-Life. For more entirely FREE tutorials and the accompanying PDFs visit http://www.handwrittentutorials.com
Просмотров: 627186 Handwritten Tutorials
Automate Immunoprecipitation and Nucleic Acid Extraction with KingFisher™ Flex purification system
 
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Learn about the system here: https://www.thermofisher.com/kingfisher The KingFisher Sample Preparation System is an integrated high throughput system that provides the highest quality nucleic acid extraction and also simplifies, standardizes, and to accelerates preparation of samples derived from various sources for downstream molecular analysis. Optimize and automate your immunoprecipitation and nucleic acid and purification workflows with Thermo Scientific™ KingFisher™ systems. KingFisher instruments reduce your hands-on time while maintaining high yields and excellent reproducibility. Optimized protocols for nucleic acids (e.g. MagMax kits), immunoprecipitation and protein purification (e.g. Dynabeads and Pierce Magnetic beads) are provided. Thermo Scientific™ Bindit™ software allows you to modify our pre-written protocols or create your own to handle more applications.
Просмотров: 971869 Thermo Fisher Scientific
Next Generation Sequencing Library Preparation - Seq It Out #10
 
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Submit your question at http://www.thermofisher.com/ask What do you call a collection of millions of DNA fragments sharing the same short sequences on the 5’ and 3’ ends? The answer is a next generation sequencing, or NGS, library. Today, we are going to focus on the four basic steps of NGS library preparation that can be broadly applied across different preparation methods. A key step in the NGS workflow is preparing the input for sequencing, known as creating a library. An NGS library is a collection of similarly sized DNA fragments with known adapter sequences added to the 5’ and 3’ ends. A library corresponds to a single sample and multiple libraries, each with their own unique adapter sequences, can be pooled and sequenced in the same sequencing run. NGS library preparation has four general steps: 1. DNA Fragmentation or Target Selection, 2. Addition of adapter sequences, 3. Size selection, and 4. Final library quantification and QC. Let’s take a look at our lab book The first step, DNA fragmentation or Target Selection. In order to get the starting DNA into smaller pieces, isolated DNA may be fragmented using physical or enzymatic methods. These libraries are referred to as fragment libraries. Alternatively, if the sequence of specific DNA targets is known, PCR amplification of those targets may be used to produce DNA amplicons within the desired size range. These libraries are referred to as amplicon libraries. Next, specific DNA adapter sequences are annealed to the 5’ and 3’ ends of the fragmented or amplicon DNA. The double-stranded DNA adapters are approximately 20 to 40bp fragments that contain known sequences. Generally, there are two different adapter sequences that can anneal to the DNA fragments in either the 5’ or 3’ orientation. One adapter sequence contains the primer annealing site for the sequencing primer, while the second adapter sequence is generally used to anchor the DNA fragment to a surface for sequencing; for example beads or a solid surface containing a complimentary DNA sequence. Now we have our DNA fragments with known adapter sequences on either end. The next step is to select the library fragment sizes we need for our sequencing run. There are two common size selection methods, the first is a gel electrophoresis based-method, while the second is bead-based size selection method. For the gel-based method, the adapted library fragments are run on a gel to separate the fragments by size and the band corresponding to the size of interest is collected. Using the bead-based method, magnetic beads are used with varying concentrations of buffers to isolate the DNA fragment sizes of interest. Final library fragment size is important for efficient, high quality DNA sequencing downstream. Bonus, when preparing amplicon libraries, size selection is usually not necessary, as long as the PCR products were already designed to be within the desired size range. We are almost done! The last and very important step is library quantification and QC. Accurate library quantification is important for successful template preparation and sequencing. There are a few library quantification methods commonly used. The first is analysis by the BioanalyzerTM system. This method gives you both library concentration and fragment size information. The second is qPCR. This method provides the most accurate library quantification information, as it only measures amplifiable library fragments, but lacks library size information. Which method you prefer is entirely up to you (and probably what’s available in your lab). And that’s NGS library preparation in a nutshell. Of course there are variations on this theme depending on your application, for example, gene expression or DNA methylation analysis, but the fundamentals stay the same. Since your library quality dictates the success of all downstream processes from template preparation to sequencing, understanding the library preparation is important to help ensure you get the highest quality sequencing data. I hope this video was helpful on NGS library, and I am sure you’ll have more questions. Submit your question at thermofisher.com/ask and subscribe to our channel to see more videos like this. And remember, when in doubt, just Seq It Out
Просмотров: 64890 Thermo Fisher Scientific
Learn about living donor kidney donation.
 
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A minimally invasive procedure for kidney removal has helped increase the number of donations by living kidney donors in the past 10 years. Laparoscopic nephrectomy requires only a few small incisions and a shorter recovery time.
Просмотров: 178841 Cleveland Clinic

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